LOCUS       40924_40957        4208 bp DNA     circular SYN 18-DEC-2018
DEFINITION  ORF selection vector pSOS plasmid pSOS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Cox JC, Lape J, Sayed MA, Hellinga HW.
  TITLE     Protein fabrication automation
  JOURNAL   Protein Sci. 16 (3), 379-390 (2007)
  PUBMED    17242375
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Cox JC, Hellinga HW.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Biochemistry, Duke University Medical 
            Center, Research Drive, DUMC 3711, Nanaline 413, Durham, NC 27710, 
            USA
REFERENCE   3  (bases 1 to 4208)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4208)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2007"; volume: "16"; issue: "3"; pages: "379-390"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2006) Biochemistry, Duke University Medical Center, Research
            Drive, DUMC 3711, Nanaline 413, Durham, NC 27710, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      70..75
                     /label=Shine-Delgarno sequence
                     /note="Shine-Delgarno sequence"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    76..83
                     /label=PacI restriction site
                     /note="PacI restriction site"
     misc_feature    92..97
                     /label=HpaI restriction site
                     /note="HpaI restriction site"
     CDS             116..769
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(846..864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(871..887)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1025..1324
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      1538..1675
                     /gene="aphA1"
                     /regulatory_class="promoter"
     CDS             1676..2467
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2677..3048
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      3189..3777
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4065..4086
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4101..4131
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4139..4155
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4163..4179
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctatagaata ctcaagctat gcatcaagct ctagtaacgg ccgccagtgt gctggaattc
       61 gcccttgtta aggagttaat taagactgct agttaacggc ggtagcggtg gctcggagaa
      121 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      181 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      241 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      301 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      361 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      421 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      481 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      541 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      601 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      661 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      721 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatgaaaggg
      781 cgaattctgc agatatccat cacactggcg gccgctcgag catgcatcta gagggcccaa
      841 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
      901 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      961 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt
     1021 acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     1081 acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg
     1141 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     1201 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     1261 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     1321 aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc ttttcacgta
     1381 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     1441 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     1501 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     1561 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttctcgc cgccaaggat
     1621 ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat
     1681 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     1741 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     1801 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     1861 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     1921 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     1981 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     2041 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     2101 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     2161 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     2221 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     2281 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     2341 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     2401 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     2461 gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     2521 gtgcggtatt tcacaccgca tacaggtggc acttttcggg gaaatgtgcg cggaacccct
     2581 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2641 taaatgcttc aataatagca cgtgaggagg gccaccatgg ccaagttgac cagtgccgtt
     2701 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     2761 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     2821 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     2881 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     2941 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     3001 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     3061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3301 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3781 gccagcaacg cggccttttt acggttcctg ggcttttgct ggccttttgc tcacatgttc
     3841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3961 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4021 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4081 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4141 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt
     4201 aggtgaca
//
